MALDI TOF MS imaging of 3D neuroblastoma cell cultures
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087892" target="_blank" >RIV/00216224:14310/16:00087892 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
MALDI TOF MS imaging of 3D neuroblastoma cell cultures
Popis výsledku v původním jazyce
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206.
Název v anglickém jazyce
MALDI TOF MS imaging of 3D neuroblastoma cell cultures
Popis výsledku anglicky
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů