Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087978" target="_blank" >RIV/00216224:14310/16:00087978 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/chromsci/bmw033" target="_blank" >http://dx.doi.org/10.1093/chromsci/bmw033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/chromsci/bmw033" target="_blank" >10.1093/chromsci/bmw033</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

Popis výsledku v původním jazyce

Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.

Název v anglickém jazyce

Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

Popis výsledku anglicky

Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-34134S" target="_blank" >GA13-34134S: Metabolizmus izoflavonů v bachoru se zaměřením na obsah equolu v kravském mléce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatographic Science

ISSN

0021-9665

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

997-1003

Kód UT WoS článku

000385022100014

EID výsledku v databázi Scopus

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