Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088379" target="_blank" >RIV/00216224:14310/16:00088379 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jbiotec.2016.07.013" target="_blank" >http://dx.doi.org/10.1016/j.jbiotec.2016.07.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jbiotec.2016.07.013" target="_blank" >10.1016/j.jbiotec.2016.07.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation

Popis výsledku v původním jazyce

To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of 251 and 243 mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.

Název v anglickém jazyce

Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation

Popis výsledku anglicky

To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of 251 and 243 mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-34134S" target="_blank" >GA13-34134S: Metabolizmus izoflavonů v bachoru se zaměřením na obsah equolu v kravském mléce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biotechnology

ISSN

0168-1656

e-ISSN

—

Svazek periodika

234

Číslo periodika v rámci svazku

september

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

43-49

Kód UT WoS článku

000382193600005

EID výsledku v databázi Scopus

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