Different motility modes in marine gregarines representing the early emerging group of Apicomplexa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088401" target="_blank" >RIV/00216224:14310/16:00088401 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Different motility modes in marine gregarines representing the early emerging group of Apicomplexa

Popis výsledku v původním jazyce

The motility of apicomplexan invasive stages (zoites), facilitated by a mechanism of substrate-dependent gliding, was investigated in important human pathogens as an essential mechanism for parasites’ migration and host cell invasion. Gregarines represent a diversified group of ancestral apicomplexans, with diverse host-parasite interactions and modes of locomotion, mechanism of which differs from apicomplexans zoites. In our research, the so called pendular or rolling movement in archigregarine Selenidium sp. was investigated after the application of drugs inducing assembly or disassembly of actin filaments (jasplakinolide, cytochalasin D) and depolymerisation of microtubules (oryzalin, colchicine). Our study confirms the influence of microtubules depolymerising drugs on Selenidium sp. motility. Despite the evidence that movement was blocked, experiments also revealed prolonged survival (90 min - 9 h) of gregarines in extremely high doses (10 micro M - 100 mM) of these probes.

Název v anglickém jazyce

Different motility modes in marine gregarines representing the early emerging group of Apicomplexa

Popis výsledku anglicky

The motility of apicomplexan invasive stages (zoites), facilitated by a mechanism of substrate-dependent gliding, was investigated in important human pathogens as an essential mechanism for parasites’ migration and host cell invasion. Gregarines represent a diversified group of ancestral apicomplexans, with diverse host-parasite interactions and modes of locomotion, mechanism of which differs from apicomplexans zoites. In our research, the so called pendular or rolling movement in archigregarine Selenidium sp. was investigated after the application of drugs inducing assembly or disassembly of actin filaments (jasplakinolide, cytochalasin D) and depolymerisation of microtubules (oryzalin, colchicine). Our study confirms the influence of microtubules depolymerising drugs on Selenidium sp. motility. Despite the evidence that movement was blocked, experiments also revealed prolonged survival (90 min - 9 h) of gregarines in extremely high doses (10 micro M - 100 mM) of these probes.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - Evropské centrum ichtyoparazitologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů