De-novo development of microsatellite markers for Dactylogyrus vistulae
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088713" target="_blank" >RIV/00216224:14310/16:00088713 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
De-novo development of microsatellite markers for Dactylogyrus vistulae
Popis výsledku v původním jazyce
In our study, we aimed to characterize de-novo microsatellite markers for Dactylogyrus parasites. Dactylogyrus vistulae was selected as a model organism owing to its relatively large body size in comparison to the other members of this genus. Approximately 300 specimens of D. vistulae were collected from European chub Squalius cephalus of the Dyje River. Genomic DNA was isolated and subsequently prepared genomic libraries were subjected to Illumina MiSeq (provided by CEITEC, MU, Brno). QDD program (Meglécz et al. 2010) was used for selecting microsatellite markers and primer design. Following criteria used in Gettová et al. (2013) 70 sequences with target microsatellites were selected. Monogenean parasites, especially dactylogyrids, display a high level of host specificity. As a result genetic structure of monogenean parasites might be host-dependent. Microsatellite markers developed for Dactylogyrus parasites might, therefore, enable us to reconstruct the evolution history of their fish hosts.
Název v anglickém jazyce
De-novo development of microsatellite markers for Dactylogyrus vistulae
Popis výsledku anglicky
In our study, we aimed to characterize de-novo microsatellite markers for Dactylogyrus parasites. Dactylogyrus vistulae was selected as a model organism owing to its relatively large body size in comparison to the other members of this genus. Approximately 300 specimens of D. vistulae were collected from European chub Squalius cephalus of the Dyje River. Genomic DNA was isolated and subsequently prepared genomic libraries were subjected to Illumina MiSeq (provided by CEITEC, MU, Brno). QDD program (Meglécz et al. 2010) was used for selecting microsatellite markers and primer design. Following criteria used in Gettová et al. (2013) 70 sequences with target microsatellites were selected. Monogenean parasites, especially dactylogyrids, display a high level of host specificity. As a result genetic structure of monogenean parasites might be host-dependent. Microsatellite markers developed for Dactylogyrus parasites might, therefore, enable us to reconstruct the evolution history of their fish hosts.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EG - Zoologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GAP505%2F12%2F0375" target="_blank" >GAP505/12/0375: Evolučně-imunitní a ekologické aspekty parazitace u hybridních a polyploidních kaprovitých ryb</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů