Real-time PCR a digital PCR v diagnostice parazitů v potravinách

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00090148" target="_blank" >RIV/00216224:14310/16:00090148 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Real-time PCR a digital PCR v diagnostice parazitů v potravinách

Popis výsledku v původním jazyce

In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation.

Název v anglickém jazyce

Real-time PCR a digital PCR in disgnostics of parasitological agents in food

Popis výsledku anglicky

In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů