Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00090237" target="_blank" >RIV/00216224:14310/16:00090237 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-016-1689-7" target="_blank" >http://dx.doi.org/10.1007/s00706-016-1689-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-016-1689-7" target="_blank" >10.1007/s00706-016-1689-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode

Popis výsledku v původním jazyce

The focus of the presented study is electrochemical investigation of DNA heptamers d(GCNNNGC) with a different center trinucleotide sequence (NNN = GAA, AAA, CCC and GGG, where A, C and G is adenine, cytosine and guanine, respectively). In phosphate-acetate buffer at pH 5.8 all heptamers were adsorbed on a hanging mercury electrode and further reduced and oxidized. The voltammetric reduction peaks of A and C moiety and oxidation peaks of G moiety at different concentrations (0.25 to 10 µmol•L-1) were analyzed. It was found that the changes in the central trinucleotide sequence of heptamers result in altered adsorption ability at a charged phase interface. An electron transfer process in an adsorptive state was evidenced by the elimination voltammetric procedure (EVP). For the evaluation of adsorption efficiency of heptamers the Langmuir adsorption model was applied and the adsorption coefficient b was calculated.

Název v anglickém jazyce

Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode

Popis výsledku anglicky

The focus of the presented study is electrochemical investigation of DNA heptamers d(GCNNNGC) with a different center trinucleotide sequence (NNN = GAA, AAA, CCC and GGG, where A, C and G is adenine, cytosine and guanine, respectively). In phosphate-acetate buffer at pH 5.8 all heptamers were adsorbed on a hanging mercury electrode and further reduced and oxidized. The voltammetric reduction peaks of A and C moiety and oxidation peaks of G moiety at different concentrations (0.25 to 10 µmol•L-1) were analyzed. It was found that the changes in the central trinucleotide sequence of heptamers result in altered adsorption ability at a charged phase interface. An electron transfer process in an adsorptive state was evidenced by the elimination voltammetric procedure (EVP). For the evaluation of adsorption efficiency of heptamers the Langmuir adsorption model was applied and the adsorption coefficient b was calculated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LH13053" target="_blank" >LH13053: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

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Svazek periodika

147

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

857-864

Kód UT WoS článku

000374172200003

EID výsledku v databázi Scopus

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