The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00090765" target="_blank" >RIV/00216224:14310/16:00090765 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
Popis výsledku v původním jazyce
The Ixodes ricinus tick is the most commonly mentioned transmitter of spirochetes belonging to the Borrelia burgdorferi sensu lato (Bbsl) group. The Bbsl complex currently contains at least 18 genospecies associated with particular reservoir hosts, 8 of which are distributed throughout Europe. The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. During 2008–2015, ticks Ixodes ricinus removed from humans were collected and tested for the presence of Bbsl. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). RealTime PCR assay based on the specific flagellin sequence amplification for the detection of B. burgdorferi s.l. was performed by EliGene Borrelia UNI kit (Elisabeth Pharmacon, Czech Republic). The kit is used in clinical laboratories in the European Union as an in vitro diagnostic medical device.
Název v anglickém jazyce
The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
Popis výsledku anglicky
The Ixodes ricinus tick is the most commonly mentioned transmitter of spirochetes belonging to the Borrelia burgdorferi sensu lato (Bbsl) group. The Bbsl complex currently contains at least 18 genospecies associated with particular reservoir hosts, 8 of which are distributed throughout Europe. The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. During 2008–2015, ticks Ixodes ricinus removed from humans were collected and tested for the presence of Bbsl. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). RealTime PCR assay based on the specific flagellin sequence amplification for the detection of B. burgdorferi s.l. was performed by EliGene Borrelia UNI kit (Elisabeth Pharmacon, Czech Republic). The kit is used in clinical laboratories in the European Union as an in vitro diagnostic medical device.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů