Molecular and biochemical characterization of cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00093421" target="_blank" >RIV/00216224:14310/16:00093421 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular and biochemical characterization of cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea)

Popis výsledku v původním jazyce

Inhibitors of cysteine peptidases (cystatins) are proteins produced by a wide range of organisms, including parasites. Besides regulation of the basic physiological functions of parasites they may act either as modulators of the host immune system or regulators of blood-digestion. We identified that cystatin genes are expressed and their protein form probably secreted by Eudiplozoon nipponicum. In following experiments we focused on molecular and functional characterization of cystatin from E. nipponicum (Monogenea). We performed bacterial expression of E. nipponicum cystatin gene (pET19b plasmid vector, E. coli BL21DE3 RIPL cells) and purified the recombinant protein [1]. Phylogenetic trees inferred using Bayesian inference, maximum likelihood, neighbour-joining methods revealed close phylogenetic relationship with cystatins of cestodes.

Název v anglickém jazyce

Molecular and biochemical characterization of cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea)

Popis výsledku anglicky

Inhibitors of cysteine peptidases (cystatins) are proteins produced by a wide range of organisms, including parasites. Besides regulation of the basic physiological functions of parasites they may act either as modulators of the host immune system or regulators of blood-digestion. We identified that cystatin genes are expressed and their protein form probably secreted by Eudiplozoon nipponicum. In following experiments we focused on molecular and functional characterization of cystatin from E. nipponicum (Monogenea). We performed bacterial expression of E. nipponicum cystatin gene (pET19b plasmid vector, E. coli BL21DE3 RIPL cells) and purified the recombinant protein [1]. Phylogenetic trees inferred using Bayesian inference, maximum likelihood, neighbour-joining methods revealed close phylogenetic relationship with cystatins of cestodes.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů