Transient suppression of gap junctional intercellular communication after exposure to 100-nanosecond pulsed electric fields
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00093524" target="_blank" >RIV/00216224:14310/16:00093524 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S1567539416300871" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1567539416300871</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bioelechem.2016.07.003" target="_blank" >10.1016/j.bioelechem.2016.07.003</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Transient suppression of gap junctional intercellular communication after exposure to 100-nanosecond pulsed electric fields
Popis výsledku v původním jazyce
Gap junctional intercellular communication (GJIC) is an important mechanism that is involved and affected in many diseases and injuries. So far, the effect of nanosecond pulsed electric fields (nsPEFs) on the communication between cells was not investigated. An in vitro approach is presented with rat liver epithelial WB-F344 cells grown and exposed in a monolayer. In order to observe sub-lethal effects, cells were exposed to pulsed electric fields with a duration of 100 ns and amplitudes between 10 and 20 kV/cm. GJIC strongly decreased within 15 min after treatment but recovered within 24 h. Gene expression of Cx43 was significantly decreased and associated with a reduced total amount of Cx43 protein. In addition, MAP kinases p38 and Erk1/2, involved in Cx43 phosphorylation, were activated and Cx43 became hyperphosphorylated. Immunofluorescent staining of Cx43 displayed the disassembly of gap junctions.
Název v anglickém jazyce
Transient suppression of gap junctional intercellular communication after exposure to 100-nanosecond pulsed electric fields
Popis výsledku anglicky
Gap junctional intercellular communication (GJIC) is an important mechanism that is involved and affected in many diseases and injuries. So far, the effect of nanosecond pulsed electric fields (nsPEFs) on the communication between cells was not investigated. An in vitro approach is presented with rat liver epithelial WB-F344 cells grown and exposed in a monolayer. In order to observe sub-lethal effects, cells were exposed to pulsed electric fields with a duration of 100 ns and amplitudes between 10 and 20 kV/cm. GJIC strongly decreased within 15 min after treatment but recovered within 24 h. Gene expression of Cx43 was significantly decreased and associated with a reduced total amount of Cx43 protein. In addition, MAP kinases p38 and Erk1/2, involved in Cx43 phosphorylation, were activated and Cx43 became hyperphosphorylated. Immunofluorescent staining of Cx43 displayed the disassembly of gap junctions.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CG - Elektrochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Bioelectrochemistry
ISSN
1567-5394
e-ISSN
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Svazek periodika
112
Číslo periodika v rámci svazku
December
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
14
Strana od-do
33-46
Kód UT WoS článku
000383523800005
EID výsledku v databázi Scopus
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