Mouse Lipocalins (MUP, OBP, LCN) Are Co-expressed in Tissues Involved in Chemical Communication

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00108174" target="_blank" >RIV/00216224:14310/16:00108174 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/16:00462823

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fevo.2016.00047/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fevo.2016.00047/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fevo.2016.00047" target="_blank" >10.3389/fevo.2016.00047</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mouse Lipocalins (MUP, OBP, LCN) Are Co-expressed in Tissues Involved in Chemical Communication

Popis výsledku v původním jazyce

Chemical communication is mediated by signal production and signal perception and in house mice (Mus musculus), both processes involve lipocalin proteins (OBP, MUP, LCN) that transport volatiles and protect them in tissues where they are produced. However, potential roles of lacrimal, nasal, and salivary lipocalins are still not well known. We aimed to determine the expression of the recently described family of odorant binding proteins (Obp), along with major urinary proteins (Mup) across different tissues in wild mice (Mus musculus) to assess the importance of these proteins based on their quantity in particular expression sites. We performed qPCR analysis of selected Mup, Lcn, Obp genes, and predicted Obp members to study their expression in selected tissues. We identified new members of the mouse odorant binding protein gene family in two subspecies, M. m. musculus and M. m. dornesticus. We show that Mup4 and Mupb from the phylogenetically older group-A are co-expressed with Obps in orofacial tissues. We also identified a sexually dimorphic pattern of female-biased Obp7 and male-biased Mup4 expression in lacrimal glands. OBPs, MUPs, and LCNs are produced in parallel, which may function to widen the spectrum of bound ligands, potentially including the degradation products of olfactory signals and/or toxic compounds. Moreover, our study demonstrates that several pheromone transporters from the lipocalin family are co-expressed in the nasal and lacrimal tissues of mice with the newly detected OBPs that further expand the already diverse mouse lipocalin family.

Název v anglickém jazyce

Mouse Lipocalins (MUP, OBP, LCN) Are Co-expressed in Tissues Involved in Chemical Communication

Popis výsledku anglicky

Chemical communication is mediated by signal production and signal perception and in house mice (Mus musculus), both processes involve lipocalin proteins (OBP, MUP, LCN) that transport volatiles and protect them in tissues where they are produced. However, potential roles of lacrimal, nasal, and salivary lipocalins are still not well known. We aimed to determine the expression of the recently described family of odorant binding proteins (Obp), along with major urinary proteins (Mup) across different tissues in wild mice (Mus musculus) to assess the importance of these proteins based on their quantity in particular expression sites. We performed qPCR analysis of selected Mup, Lcn, Obp genes, and predicted Obp members to study their expression in selected tissues. We identified new members of the mouse odorant binding protein gene family in two subspecies, M. m. musculus and M. m. dornesticus. We show that Mup4 and Mupb from the phylogenetically older group-A are co-expressed with Obps in orofacial tissues. We also identified a sexually dimorphic pattern of female-biased Obp7 and male-biased Mup4 expression in lacrimal glands. OBPs, MUPs, and LCNs are produced in parallel, which may function to widen the spectrum of bound ligands, potentially including the degradation products of olfactory signals and/or toxic compounds. Moreover, our study demonstrates that several pheromone transporters from the lipocalin family are co-expressed in the nasal and lacrimal tissues of mice with the newly detected OBPs that further expand the already diverse mouse lipocalin family.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FRONTIERS IN ECOLOGY AND EVOLUTION

ISSN

2296-701X

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

2016

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

000517761700047

EID výsledku v databázi Scopus

2-s2.0-85002222228