Study of red clover (Trifolium pratense L.) genes regulating nitrogen fixation and nodulation process

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00094416" target="_blank" >RIV/00216224:14310/17:00094416 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of red clover (Trifolium pratense L.) genes regulating nitrogen fixation and nodulation process

Popis výsledku v původním jazyce

Symbiotic nitrogen fixation is a mutualistic bacteria-plant relationship in which bacteria provides nitrogen converted into organic compounds and plant supplies the bacteria with carbon and energy source. The best known and the most important symbiotic relationship occurs between plants of the family Fabaceae and rhizobacteria. Efficiency of symbiotic nitrogen fixation differs between plant species but also varies within species. This phenomenon has been described as the result of polymorphisms in quantitative trait loci associated with symbiotic nitrogen fixation. Our study was focused to finding associations between level of nitrogen fixation and candidate genes for this ability for further application in red clover breeding. Computational analysis included in silico identification of 17 candidate legume genes for controlling the nodulation process (CLE12, CRE1, DMI3, DNF2, EFD, ERN, LYK3, NFP, NIN, NSP1, NSP2, PEN3, PHO2, PNO1, RDN1, SKL1, SUNN) and sequence capturing from the assembled genome of M. truncatula, T. pratense and T. subterraneum for multiple alignment and searching conserved regions for primer design. Secondly, large population of red clover genotypes was determined for nitrogen fixation ability by acetylene reduction assay for nitrogenase activity. Forty-eight plants (varieties Start, Global, Columbia and Vltavín) efficient and inefficient in nitrogen fixation were used for DNA isolation and comparative analyses using The Roche NimbleGen SeqCap EZ enrichment system to enrich targeted regions (100 kb in total) in the candidate genes. Enriched gene regions are intended to be sequenced on Illumina MiSeq System with the coverage 2000x. The sequence data will be then used for SNP mining and association studies between obtained phenotypes of red clover contrast for nitrogen fixation ability.

Název v anglickém jazyce

Study of red clover (Trifolium pratense L.) genes regulating nitrogen fixation and nodulation process

Popis výsledku anglicky

Symbiotic nitrogen fixation is a mutualistic bacteria-plant relationship in which bacteria provides nitrogen converted into organic compounds and plant supplies the bacteria with carbon and energy source. The best known and the most important symbiotic relationship occurs between plants of the family Fabaceae and rhizobacteria. Efficiency of symbiotic nitrogen fixation differs between plant species but also varies within species. This phenomenon has been described as the result of polymorphisms in quantitative trait loci associated with symbiotic nitrogen fixation. Our study was focused to finding associations between level of nitrogen fixation and candidate genes for this ability for further application in red clover breeding. Computational analysis included in silico identification of 17 candidate legume genes for controlling the nodulation process (CLE12, CRE1, DMI3, DNF2, EFD, ERN, LYK3, NFP, NIN, NSP1, NSP2, PEN3, PHO2, PNO1, RDN1, SKL1, SUNN) and sequence capturing from the assembled genome of M. truncatula, T. pratense and T. subterraneum for multiple alignment and searching conserved regions for primer design. Secondly, large population of red clover genotypes was determined for nitrogen fixation ability by acetylene reduction assay for nitrogenase activity. Forty-eight plants (varieties Start, Global, Columbia and Vltavín) efficient and inefficient in nitrogen fixation were used for DNA isolation and comparative analyses using The Roche NimbleGen SeqCap EZ enrichment system to enrich targeted regions (100 kb in total) in the candidate genes. Enriched gene regions are intended to be sequenced on Illumina MiSeq System with the coverage 2000x. The sequence data will be then used for SNP mining and association studies between obtained phenotypes of red clover contrast for nitrogen fixation ability.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/TH02010351" target="_blank" >TH02010351: Efektivní biotechnologie pro syntézu populačních čipů a její ověření na pilotní matrici rostlinného materiálu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů