Cold plasma: The way to improve the SALD ICP MS analysis repeatibility

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00095326" target="_blank" >RIV/00216224:14310/17:00095326 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cold plasma: The way to improve the SALD ICP MS analysis repeatibility

Popis výsledku v původním jazyce

This work deals with the preparation of model biological microsamples for Substrate Assisted Laser Desorption Inductively Coupled Plasma Mass Spectrometry (SALD ICP MS). This technique provides direct and fast analysis of liquid samples deposited onto polyethylene terephthalate (PET) plate (substrate) in the form of dried droplets with minimal sample preparation and submicrolitre sample volume requirements. Furthermore, SALD allows direct analysis of samples in organic solvents, which cannot be directly nebulised to ICP MS. These benefits are, however, balanced out by low repeatability of the assay, typically about 30 %. One of the approaches to increasing the repeatability is a modification of the substrate surface by Diffuse Coplanar Surface Barrier Discharge (DCSBD) with an addition of an internal standard to the sample. Using this approach, a relative standard deviation under 10 % for model biological samples can be achieved. The only limitation of this technique is the occurrence of the so-called “aging effect”, i.e., the gradual return of the modified physicochemical surface properties to the original state.

Název v anglickém jazyce

Cold plasma: The way to improve the SALD ICP MS analysis repeatibility

Popis výsledku anglicky

This work deals with the preparation of model biological microsamples for Substrate Assisted Laser Desorption Inductively Coupled Plasma Mass Spectrometry (SALD ICP MS). This technique provides direct and fast analysis of liquid samples deposited onto polyethylene terephthalate (PET) plate (substrate) in the form of dried droplets with minimal sample preparation and submicrolitre sample volume requirements. Furthermore, SALD allows direct analysis of samples in organic solvents, which cannot be directly nebulised to ICP MS. These benefits are, however, balanced out by low repeatability of the assay, typically about 30 %. One of the approaches to increasing the repeatability is a modification of the substrate surface by Diffuse Coplanar Surface Barrier Discharge (DCSBD) with an addition of an internal standard to the sample. Using this approach, a relative standard deviation under 10 % for model biological samples can be achieved. The only limitation of this technique is the occurrence of the so-called “aging effect”, i.e., the gradual return of the modified physicochemical surface properties to the original state.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CECE 2017 14th International Interdisciplinary Meeting on Bioanalysis

ISBN

9789633961032

ISSN

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e-ISSN

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Počet stran výsledku

167

Strana od-do

143-147

Název nakladatele

MTA-PE Translational Glycomics Research Group, Research Institute for Biomolecular and Chemical Engineering, University of Pannonia Egyetem u. 10, H-8200 Veszprém, Hungary

Místo vydání

Veszprém, Hungary

Místo konání akce

Veszprém, Hungary

Datum konání akce

8. 10. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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