FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00100422" target="_blank" >RIV/00216224:14310/17:00100422 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.cellsig.2017.01.023" target="_blank" >http://dx.doi.org/10.1016/j.cellsig.2017.01.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cellsig.2017.01.023" target="_blank" >10.1016/j.cellsig.2017.01.023</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis
Popis výsledku v původním jazyce
Among the 10 Frizzled (FZD) isoforms belonging to the Class F of G protein-coupled receptors (GPCRs), FZD(10) remains the most enigmatic. FZD10 shows homology to FZD(4) and FZD(9) and was previously implicated in both beta-catenin-dependent and-independent signalling. In normal tissue, FZD(10) levels are generally very low; however, its upregulation in synovial carcinoma has attracted some attention for therapy. Our findings identify FZD(10), as a receptor interacting with and signalling through the heterotrimeric G protein G alpha(13) but not G alpha(12), G alpha(i1,) G alpha(oA), G alpha(s), or G alpha(q). Stimulation with the FZD agonist WNT induced the dissociation of the G alpha(13) protein from FZD(10), and led to global G alpha(12/13)-dependent cell changes assessed by dynamic mass redistribution measurements. Furthermore, we show that FZD(10) mediates G alpha(12/13) activation-dependent induction of YAP/TAZ transcriptional activity. In addition, we show a distinct expression of FZD(10) in embryonic CNS endothelial cells at E11.5-E14.5. Given the well-known importance of G alpha(13) signalling for the development of the vascular system, the selective expression of FZD(10) in brain vascular endothelial cells points at a potential role of FZD(10)-G alpha(13) signalling in CNS angiogenesis. (C) 2017 Elsevier Inc. All rights reserved.
Název v anglickém jazyce
FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis
Popis výsledku anglicky
Among the 10 Frizzled (FZD) isoforms belonging to the Class F of G protein-coupled receptors (GPCRs), FZD(10) remains the most enigmatic. FZD10 shows homology to FZD(4) and FZD(9) and was previously implicated in both beta-catenin-dependent and-independent signalling. In normal tissue, FZD(10) levels are generally very low; however, its upregulation in synovial carcinoma has attracted some attention for therapy. Our findings identify FZD(10), as a receptor interacting with and signalling through the heterotrimeric G protein G alpha(13) but not G alpha(12), G alpha(i1,) G alpha(oA), G alpha(s), or G alpha(q). Stimulation with the FZD agonist WNT induced the dissociation of the G alpha(13) protein from FZD(10), and led to global G alpha(12/13)-dependent cell changes assessed by dynamic mass redistribution measurements. Furthermore, we show that FZD(10) mediates G alpha(12/13) activation-dependent induction of YAP/TAZ transcriptional activity. In addition, we show a distinct expression of FZD(10) in embryonic CNS endothelial cells at E11.5-E14.5. Given the well-known importance of G alpha(13) signalling for the development of the vascular system, the selective expression of FZD(10) in brain vascular endothelial cells points at a potential role of FZD(10)-G alpha(13) signalling in CNS angiogenesis. (C) 2017 Elsevier Inc. All rights reserved.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/EE2.3.20.0180" target="_blank" >EE2.3.20.0180: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cellular Signalling
ISSN
0898-6568
e-ISSN
—
Svazek periodika
32
Číslo periodika v rámci svazku
April
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
93-103
Kód UT WoS článku
000395953400010
EID výsledku v databázi Scopus
—