Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00108732" target="_blank" >RIV/00216224:14310/17:00108732 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/17:N0000024

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418695/pdf/13567_2017_Article_433.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418695/pdf/13567_2017_Article_433.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13567-017-0433-6" target="_blank" >10.1186/s13567-017-0433-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Popis výsledku v původním jazyce

Porcine reproductive and respiratory syndrome (PRRS) is one of the most signifcant and economically important infectious diseases afecting swine worldwide and can predispose pigs to secondary bacterial infections caused by, e.g. Haemophilus parasuis. The aim of the presented study was to compare susceptibility of two diferent types of macrophages which could be in contact with both pathogens during infection with PRRS virus (PRRSV) and in co-infection with H. parasuis. Alveolar macrophages (PAMs) as resident cells provide one of the frst lines of defence against microbes invading lung tissue. On the other hand, monocyte derived macrophages (MDMs) represent infammatory cells accumulating at the site of infammation. While PAMs were relatively resistant to cytopathogenic efect caused by PRRSV, MDMs were much more sensitive to PRRSV infection. MDMs infected with PRRSV increased expression of pro-apoptotic Bad, Bax and p53 mRNA. Increased mortality of MDMs may be also related to a higher intensity of ROS production after infection with PRRSV. In addition, MDMs (but not PAMs) infected with H. parasuis alone formed multinucleated giant cells (MGC); these cells were not observed in MDMs infected with both pathogens. Higher sensitivity of MDMs to PRRSV infection, which is associated with limited MDMs survival and restriction of MGC formation, could contribute to the development of multifactorial respiratory disease of swine.

Název v anglickém jazyce

Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Popis výsledku anglicky

Porcine reproductive and respiratory syndrome (PRRS) is one of the most signifcant and economically important infectious diseases afecting swine worldwide and can predispose pigs to secondary bacterial infections caused by, e.g. Haemophilus parasuis. The aim of the presented study was to compare susceptibility of two diferent types of macrophages which could be in contact with both pathogens during infection with PRRS virus (PRRSV) and in co-infection with H. parasuis. Alveolar macrophages (PAMs) as resident cells provide one of the frst lines of defence against microbes invading lung tissue. On the other hand, monocyte derived macrophages (MDMs) represent infammatory cells accumulating at the site of infammation. While PAMs were relatively resistant to cytopathogenic efect caused by PRRSV, MDMs were much more sensitive to PRRSV infection. MDMs infected with PRRSV increased expression of pro-apoptotic Bad, Bax and p53 mRNA. Increased mortality of MDMs may be also related to a higher intensity of ROS production after infection with PRRSV. In addition, MDMs (but not PAMs) infected with H. parasuis alone formed multinucleated giant cells (MGC); these cells were not observed in MDMs infected with both pathogens. Higher sensitivity of MDMs to PRRSV infection, which is associated with limited MDMs survival and restriction of MGC formation, could contribute to the development of multifactorial respiratory disease of swine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Research

ISSN

0928-4249

e-ISSN

1297-9716

Svazek periodika

48

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

28

Kód UT WoS článku

000401466700001

EID výsledku v databázi Scopus

2-s2.0-85018769465