The forebody organisation in Paradiplozoon homoion: A microscopic study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00100949" target="_blank" >RIV/00216224:14310/18:00100949 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The forebody organisation in Paradiplozoon homoion: A microscopic study

Popis výsledku v původním jazyce

The family Diplozoidae (Monogenea), including investigated species Paradiplozoon homoion, represent blood-feeding gill ectoparasites of freshwater fish. They exhibit unusual body organisation and life cycle involving a permanent fusion of two larval worms and their subsequent transformation into one individual. Using a combined approach of confocal and electron microscopy, this study focused on the parasite forebody. Special attention was given to parasite’s nervous, excretory and digestive systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers and the pharynx. Staining with chloride carmine or counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.

Název v anglickém jazyce

The forebody organisation in Paradiplozoon homoion: A microscopic study

Popis výsledku anglicky

The family Diplozoidae (Monogenea), including investigated species Paradiplozoon homoion, represent blood-feeding gill ectoparasites of freshwater fish. They exhibit unusual body organisation and life cycle involving a permanent fusion of two larval worms and their subsequent transformation into one individual. Using a combined approach of confocal and electron microscopy, this study focused on the parasite forebody. Special attention was given to parasite’s nervous, excretory and digestive systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers and the pharynx. Staining with chloride carmine or counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - Evropské centrum ichtyoparazitologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů