MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00101298" target="_blank" >RIV/00216224:14310/18:00101298 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s

Popis výsledku v původním jazyce

The practicality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) applied to molecular imaging of biological tissues is limited by the analysis speed. A relatively low-speed motion of xy stages is one of the factors reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging time-of-flight (TOF) mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser beam repositioning on a target plate. [1] The laser beam was focused via a modified grid into a 10 um round spot. The modified optical system of our MALDI TOF mass spectrometer allowed the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates up to ~150 pixels/s. The influence of selected parameters on the total MS imaging time was examined. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates. A single MS image comprising 100 × 100 pixels with 10 um lateral resolution was recorded in approximately 70 seconds.

Název v anglickém jazyce

MALDI mass spectrometry imaging at acquisition rates above 100 pixel/s

Popis výsledku anglicky

The practicality of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) applied to molecular imaging of biological tissues is limited by the analysis speed. A relatively low-speed motion of xy stages is one of the factors reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging time-of-flight (TOF) mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser beam repositioning on a target plate. [1] The laser beam was focused via a modified grid into a 10 um round spot. The modified optical system of our MALDI TOF mass spectrometer allowed the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates up to ~150 pixels/s. The influence of selected parameters on the total MS imaging time was examined. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates. A single MS image comprising 100 × 100 pixels with 10 um lateral resolution was recorded in approximately 70 seconds.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA15-05387S" target="_blank" >GA15-05387S: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CECE 2018 15th International Interdisciplinary Meeting on Bioanalysis

ISBN

9788090495951

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

19,33

Název nakladatele

Institute of Analytical Chemistry of the CAS, v. v. i.

Místo vydání

Brno, Czech Republic

Místo konání akce

Brno

Datum konání akce

1. 1. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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