Immunomodulatory effects of selected cyanobacterial peptides in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00101600" target="_blank" >RIV/00216224:14310/18:00101600 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/18:00491690

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0041010118301740" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0041010118301740</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxicon.2018.04.031" target="_blank" >10.1016/j.toxicon.2018.04.031</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunomodulatory effects of selected cyanobacterial peptides in vitro

Popis výsledku v původním jazyce

Cyanobacteria produce many biologically active metabolites synthesized via nonribosomal synthetic pathways such as cyclic microcystins (MCs) and linear aeruginosins (Aers). The present study aimed to investigate the effects of different MC variants and the newly isolated aerugenosin Aer-865 on macrophages, which represent one of the key effector cells within the innate immune responses. Specifically, our study included RAW 264.7 macrophage activation associated with production of cytotoxic and cytostatic nitric oxide (NO) as well as pro-inflammatory mediators like tumor necrosis factor alpha (TNF alpha) and interleukin 6 (IL-6). From the compounds investigated, commonly occurring MC variants (-RR,-YR) and Aer-865 had no significant effects within the non-cytotoxic concentrations tested, i.e. 0.001-1 mu M for MCs and 0.1-50 mu M for Aer-865. In contrast to known immunoactive MC-LR, the negligible immunomodulatory potential of tested MC congeners could be related to their differences in structure. The knowledge of MC structure-specific activities contributes to the understanding of complex toxicity of different MC variants and most importantly their mixtures. This study is one of the first study that evaluate the effect of larger set of cyanobacterial peptides on macrophages and compare their immunomodulatory potential.

Název v anglickém jazyce

Immunomodulatory effects of selected cyanobacterial peptides in vitro

Popis výsledku anglicky

Cyanobacteria produce many biologically active metabolites synthesized via nonribosomal synthetic pathways such as cyclic microcystins (MCs) and linear aeruginosins (Aers). The present study aimed to investigate the effects of different MC variants and the newly isolated aerugenosin Aer-865 on macrophages, which represent one of the key effector cells within the innate immune responses. Specifically, our study included RAW 264.7 macrophage activation associated with production of cytotoxic and cytostatic nitric oxide (NO) as well as pro-inflammatory mediators like tumor necrosis factor alpha (TNF alpha) and interleukin 6 (IL-6). From the compounds investigated, commonly occurring MC variants (-RR,-YR) and Aer-865 had no significant effects within the non-cytotoxic concentrations tested, i.e. 0.001-1 mu M for MCs and 0.1-50 mu M for Aer-865. In contrast to known immunoactive MC-LR, the negligible immunomodulatory potential of tested MC congeners could be related to their differences in structure. The knowledge of MC structure-specific activities contributes to the understanding of complex toxicity of different MC variants and most importantly their mixtures. This study is one of the first study that evaluate the effect of larger set of cyanobacterial peptides on macrophages and compare their immunomodulatory potential.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicon

ISSN

0041-0101

e-ISSN

—

Svazek periodika

149

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

20-25

Kód UT WoS článku

000436917900003

EID výsledku v databázi Scopus

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