Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00104762" target="_blank" >RIV/00216224:14310/18:00104762 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0011224018302001" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0011224018302001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2018.10.004" target="_blank" >10.1016/j.cryobiol.2018.10.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence

Popis výsledku v původním jazyce

The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ross Island, Antarctica. Culture of Heterococcus sp. grown in liquid medium were used to study ribitol effects at sub-zero temperatures on the species resistance to rapid freezing (RF, immersion of a sample into liquid nitrogen) and consequent cultivation on agar. Before the experiment, Heterococcus sp. was cultured in liquid medium for 11 months and then treated in ribitol concentrations of 32 or 50 mM for 2h. Then, 1ml samples were frozen to minus 196°C in liquid nitrogen (day 0) and inoculated on BBM agar after thawing. Number of living and dead cells was evaluated and the cell viability (PV was calculated repeatedly using the optical microscopy approach. The addition of ribitol caused a noticable increase in PV on days 9, 12, 14 (with a PV of 25–45% in ribitol-treated samples compared to 10% in the untreated control). In the following period (d 16–19), the positive effect of ribitol on Pv was less pronounced but still statistically significant. To evaluate the negative effects of RF on chlorophyll fluorescence parameters, the potential yield of photochemical reactions in PS II (FV/FM), and the effective quantum yield of photochemical reactions in PS II (PhiPSII) were measured immediately before and after RF. Consequently, FV/FM and PhiPSII of agar inoculates were measured repeatedly for 30 d cultivation in 3 d interval. Both..

Název v anglickém jazyce

Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence

Popis výsledku anglicky

The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ross Island, Antarctica. Culture of Heterococcus sp. grown in liquid medium were used to study ribitol effects at sub-zero temperatures on the species resistance to rapid freezing (RF, immersion of a sample into liquid nitrogen) and consequent cultivation on agar. Before the experiment, Heterococcus sp. was cultured in liquid medium for 11 months and then treated in ribitol concentrations of 32 or 50 mM for 2h. Then, 1ml samples were frozen to minus 196°C in liquid nitrogen (day 0) and inoculated on BBM agar after thawing. Number of living and dead cells was evaluated and the cell viability (PV was calculated repeatedly using the optical microscopy approach. The addition of ribitol caused a noticable increase in PV on days 9, 12, 14 (with a PV of 25–45% in ribitol-treated samples compared to 10% in the untreated control). In the following period (d 16–19), the positive effect of ribitol on Pv was less pronounced but still statistically significant. To evaluate the negative effects of RF on chlorophyll fluorescence parameters, the potential yield of photochemical reactions in PS II (FV/FM), and the effective quantum yield of photochemical reactions in PS II (PhiPSII) were measured immediately before and after RF. Consequently, FV/FM and PhiPSII of agar inoculates were measured repeatedly for 30 d cultivation in 3 d interval. Both..

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cryobiology

ISSN

0011-2240

e-ISSN

1090-2392

Svazek periodika

85

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

39-46

Kód UT WoS článku

000454851300007

EID výsledku v databázi Scopus

2-s2.0-85055909602