The oxidation double peak of reduced guanine residues in short oligodeoxynucleotides: A study of its origin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00106524" target="_blank" >RIV/00216224:14310/18:00106524 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.jelechem.2018.03.013" target="_blank" >https://doi.org/10.1016/j.jelechem.2018.03.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.013" target="_blank" >10.1016/j.jelechem.2018.03.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The oxidation double peak of reduced guanine residues in short oligodeoxynucleotides: A study of its origin

Popis výsledku v původním jazyce

The aim of this study is to find a deeper understanding of the long neglected oxidation double peak of reduced guanines (G(red)ODP) produced at a mercury electrode (ME) by the reverse oxidation of the guanine reduction product (G(red)). Although the reduction processes of G moieties at an ME are hidden in the cathodic currents of the background solvent decompositions, and the G reduction process thus cannot be examined directly, the G reduction product can be oxidized back to G, yielding an oxidation signal at about 0.2V. depending on the measurement conditions, the oxidation signal of Gred may assume the form of a single peak or a double peak with GI and GII. The cause of the double peak generation remains unknown. However, the novel approach based on combining elements of chronoamperometry and voltammetry proposes that the G(red)ODP originates from the oxidation of DNA fragments in two different adsorption conformations. The results indicate thet the orientation of G residues is controlled by the repulsive interaction after thier reduction and from the difference of GI and GII potentials the repulsion energy was estimated. The new findings may find application as a tool to indicate the structural and surface properties of short oligonucleotides with G bases

Název v anglickém jazyce

The oxidation double peak of reduced guanine residues in short oligodeoxynucleotides: A study of its origin

Popis výsledku anglicky

The aim of this study is to find a deeper understanding of the long neglected oxidation double peak of reduced guanines (G(red)ODP) produced at a mercury electrode (ME) by the reverse oxidation of the guanine reduction product (G(red)). Although the reduction processes of G moieties at an ME are hidden in the cathodic currents of the background solvent decompositions, and the G reduction process thus cannot be examined directly, the G reduction product can be oxidized back to G, yielding an oxidation signal at about 0.2V. depending on the measurement conditions, the oxidation signal of Gred may assume the form of a single peak or a double peak with GI and GII. The cause of the double peak generation remains unknown. However, the novel approach based on combining elements of chronoamperometry and voltammetry proposes that the G(red)ODP originates from the oxidation of DNA fragments in two different adsorption conformations. The results indicate thet the orientation of G residues is controlled by the repulsive interaction after thier reduction and from the difference of GI and GII potentials the repulsion energy was estimated. The new findings may find application as a tool to indicate the structural and surface properties of short oligonucleotides with G bases

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/LD15058" target="_blank" >LD15058: Příprava substrátů pro povrchově zesílenou Ramanovu spektroskopii pomocí elektrochemických, elektroforetických a jiskrový výboj využívajících technik.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

1873-2569

Svazek periodika

821

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

104-110

Kód UT WoS článku

000437818600017

EID výsledku v databázi Scopus

2-s2.0-85046100800