Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00109166" target="_blank" >RIV/00216224:14310/18:00109166 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652079:_____/18:00490877

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11240-018-1441-6" target="_blank" >https://link.springer.com/article/10.1007/s11240-018-1441-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-018-1441-6" target="_blank" >10.1007/s11240-018-1441-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study

Popis výsledku v původním jazyce

Photoautotrophic (PA) suspension cultures combine the advantages of cell suspensions with carbon autotrophy and have been used for various applications including studies on photosynthesis, sugar signal transduction, herbicide action, and stress responses. A major practical drawback is the requirement for elevated CO2, which is typically generated by a buffer system in two-tier flasks that need to be custom made and require time-consuming handling. In this study, we substantially simplified and improved the cultivation of a PA culture of Chenopodium rubrum as a case study in Erlenmeyer flasks shaken in a CO2 enriched photoincubator. Growth rates and photosynthetic activity were found to be comparable to those in two-tier flasks but with the advantage of constant CO2 level throughout the cultivation. In addition, it was possible to establish the cultivation in a commercial laboratory-scale photobioreactor with various options to control and continuously measure the culture conditions and regimes and assess the physiological status. Although the relative biomass yield and photosynthetic performance of the batch cultures was not fully reached, the continuous cultivation system provides a good basis for future scale-up if larger amounts of biomass are needed or to impose specific cultivation regimes in combination with comprehensive online monitoring. The different improvements should contribute to the more widespread use of higher plant PA cultures and also facilitate the cultivation of microalgae.

Název v anglickém jazyce

Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study

Popis výsledku anglicky

Photoautotrophic (PA) suspension cultures combine the advantages of cell suspensions with carbon autotrophy and have been used for various applications including studies on photosynthesis, sugar signal transduction, herbicide action, and stress responses. A major practical drawback is the requirement for elevated CO2, which is typically generated by a buffer system in two-tier flasks that need to be custom made and require time-consuming handling. In this study, we substantially simplified and improved the cultivation of a PA culture of Chenopodium rubrum as a case study in Erlenmeyer flasks shaken in a CO2 enriched photoincubator. Growth rates and photosynthetic activity were found to be comparable to those in two-tier flasks but with the advantage of constant CO2 level throughout the cultivation. In addition, it was possible to establish the cultivation in a commercial laboratory-scale photobioreactor with various options to control and continuously measure the culture conditions and regimes and assess the physiological status. Although the relative biomass yield and photosynthetic performance of the batch cultures was not fully reached, the continuous cultivation system provides a good basis for future scale-up if larger amounts of biomass are needed or to impose specific cultivation regimes in combination with comprehensive online monitoring. The different improvements should contribute to the more widespread use of higher plant PA cultures and also facilitate the cultivation of microalgae.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Projekt

<a href="/cs/project/LM2015055" target="_blank" >LM2015055: Centrum pro systémovou biologii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

135

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

37-51

Kód UT WoS článku

000444955700004

EID výsledku v databázi Scopus

2-s2.0-85048746326