Cytokinin signalling regulates organ identity via AHK4 receptor in Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00118735" target="_blank" >RIV/00216224:14310/18:00118735 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinin signalling regulates organ identity via AHK4 receptor in Arabidopsis

Popis výsledku v původním jazyce

Plants are able to undergo postembryonic de novo organogenesis and thus effectively regenerate damaged tissues. Root or shoot identity of newly formed organs depends on mutual interactions of the phytohormones cytokinins and auxin. However, our understanding to the role of hormonal regulations in the cell fate reprograming is still elusive. In the hypocotyl explant assay, auxin activated root formation while cytokinins mediated early loss of the root identity, disorganization of primordia structure and initiation of shoot development. Exogenous but also endogenous cytokinins influenced both the initiation of newly formed organs as well as the pace of organ developmental sequence. The process of de novo shoot apical meristem establishment was accompanied by strong activation of AHK4-mediated cytokinin signalling, induction of shoot-specific homeodomain regulator WUSCHEL specifically in the disorganized primordia and upregulation of endogenous isopentenyladenine-type cytokinins. Moreover, AHK4-controlled cytokinin signalling negatively regulates root stem cell organizer WUSCHEL RELATED HOMEOBOX 5 in the root quiescent centre. We propose an important role of endogenous cytokinin biosynthesis and AHK4-mediated cytokinin signalling in the control of de novo induced organ identity.

Název v anglickém jazyce

Cytokinin signalling regulates organ identity via AHK4 receptor in Arabidopsis

Popis výsledku anglicky

Plants are able to undergo postembryonic de novo organogenesis and thus effectively regenerate damaged tissues. Root or shoot identity of newly formed organs depends on mutual interactions of the phytohormones cytokinins and auxin. However, our understanding to the role of hormonal regulations in the cell fate reprograming is still elusive. In the hypocotyl explant assay, auxin activated root formation while cytokinins mediated early loss of the root identity, disorganization of primordia structure and initiation of shoot development. Exogenous but also endogenous cytokinins influenced both the initiation of newly formed organs as well as the pace of organ developmental sequence. The process of de novo shoot apical meristem establishment was accompanied by strong activation of AHK4-mediated cytokinin signalling, induction of shoot-specific homeodomain regulator WUSCHEL specifically in the disorganized primordia and upregulation of endogenous isopentenyladenine-type cytokinins. Moreover, AHK4-controlled cytokinin signalling negatively regulates root stem cell organizer WUSCHEL RELATED HOMEOBOX 5 in the root quiescent centre. We propose an important role of endogenous cytokinin biosynthesis and AHK4-mediated cytokinin signalling in the control of de novo induced organ identity.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů