Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00110709" target="_blank" >RIV/00216224:14310/19:00110709 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf" target="_blank" >http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis
Popis výsledku v původním jazyce
Diagnosis of tuberculosis from historical specimens has been mostly based on detection of IS6110 by PCR and following gel electrophoresis. Even when some authors stressed the possibility that this insertion sequence may have similar counterparts in other microorganisms (e.g. soil bacteria), it continued to be used widely, sometimes without sequencing of the PCR product. This work is aiming at IS6110 and two other sequences that are potential targets for DNA detection of Mycobacteria tuberculosis complex. These three loci are compared by bioinformatic and phylogenetic analysis to assess their usability in ancient DNA studies.
Název v anglickém jazyce
Need for novel detection targets in tuberculosis diagnostics of historical specimens - bioinformatic analysis
Popis výsledku anglicky
Diagnosis of tuberculosis from historical specimens has been mostly based on detection of IS6110 by PCR and following gel electrophoresis. Even when some authors stressed the possibility that this insertion sequence may have similar counterparts in other microorganisms (e.g. soil bacteria), it continued to be used widely, sometimes without sequencing of the PCR product. This work is aiming at IS6110 and two other sequences that are potential targets for DNA detection of Mycobacteria tuberculosis complex. These three loci are compared by bioinformatic and phylogenetic analysis to assess their usability in ancient DNA studies.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů