LONELY GUY is not alone

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00124860" target="_blank" >RIV/00216224:14310/20:00124860 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

LONELY GUY is not alone

Popis výsledku v původním jazyce

Development through the reiterative organogenesis is common in eukaryote development. In plants, new organs are periodically initiated in a regular pattern called phyllotaxis. Self-organization of phyllotaxis is driven by spatio-temporal distribution of the hormone auxin and the temporal robustness of the pattern is also under the influence of another hormone, cytokinin. However, the molecular mechanisms underlying cytokinin action in the temporal pattern of reiterative organ production are unknown. We have found using expression analysis that several cytokinin biosynthetic enzymes from the LONELY GUY family (LOGs) are potentially upregulated by auxin in Arabidopsis. We have analyzed the expression of all LOGs in the shoot apical meristem, using proLOG-GFP lines, and identified several LOGs differentially expressed in the meristem. To explore in more detail how the LOGs are regulated, we are in addition using synthetic biology tools to understand quantitatively the dynamics of their response to auxin. This approach will allow to further understand how auxin-cytokinin cross-regulation participates to the dynamics of shoot apical meristem patterning.

Název v anglickém jazyce

LONELY GUY is not alone

Popis výsledku anglicky

Development through the reiterative organogenesis is common in eukaryote development. In plants, new organs are periodically initiated in a regular pattern called phyllotaxis. Self-organization of phyllotaxis is driven by spatio-temporal distribution of the hormone auxin and the temporal robustness of the pattern is also under the influence of another hormone, cytokinin. However, the molecular mechanisms underlying cytokinin action in the temporal pattern of reiterative organ production are unknown. We have found using expression analysis that several cytokinin biosynthetic enzymes from the LONELY GUY family (LOGs) are potentially upregulated by auxin in Arabidopsis. We have analyzed the expression of all LOGs in the shoot apical meristem, using proLOG-GFP lines, and identified several LOGs differentially expressed in the meristem. To explore in more detail how the LOGs are regulated, we are in addition using synthetic biology tools to understand quantitatively the dynamics of their response to auxin. This approach will allow to further understand how auxin-cytokinin cross-regulation participates to the dynamics of shoot apical meristem patterning.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/EF17_050%2F0008496" target="_blank" >EF17_050/0008496: MSCAfellow@MUNI</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů