Changes of peptidase profile during Fasciola hepatica embryonation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00119646" target="_blank" >RIV/00216224:14310/21:00119646 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Changes of peptidase profile during Fasciola hepatica embryonation

Popis výsledku v původním jazyce

Fasciola hepatica is a widespread foodborne trematode that parasitizes a broad range of mammals, including humans. Adults of this cosmopolitan species lay their propagation stages - unsegmented operculated eggs, into the bile duct of their mammalian host. Crucial aspects of F. hepatica life cycle, such as migration, reproduction and immune evasion, are directly dependent on the activity of proteolytic enzymes, also known as peptidases. Expression of these molecules have been intensely studied in metacercariae, newly excysted juveniles and adults, however embryonic stages of F. hepatica, have not yielded much scientific attention in this context. We used combined transcriptomic/proteomic approach which led to the identification of expressed and translated peptidases in developing eggs of different age. Additionally, activity profiling with the substrate library and selective inhibitors identified presence of different peptidase classes in egg extracts. Our results indicate that F. hepatica egg transcriptome remains mostly constant, while the proteome is being considerably adjusted throughout the embryonation. Threonine catalytic subunits of proteasome are constantly produced, while the cysteine peptidases prevailing in freshly laid eggs are supplemented by aspartic peptidases and metallopeptidases in the later stages of egg development.

Název v anglickém jazyce

Changes of peptidase profile during Fasciola hepatica embryonation

Popis výsledku anglicky

Fasciola hepatica is a widespread foodborne trematode that parasitizes a broad range of mammals, including humans. Adults of this cosmopolitan species lay their propagation stages - unsegmented operculated eggs, into the bile duct of their mammalian host. Crucial aspects of F. hepatica life cycle, such as migration, reproduction and immune evasion, are directly dependent on the activity of proteolytic enzymes, also known as peptidases. Expression of these molecules have been intensely studied in metacercariae, newly excysted juveniles and adults, however embryonic stages of F. hepatica, have not yielded much scientific attention in this context. We used combined transcriptomic/proteomic approach which led to the identification of expressed and translated peptidases in developing eggs of different age. Additionally, activity profiling with the substrate library and selective inhibitors identified presence of different peptidase classes in egg extracts. Our results indicate that F. hepatica egg transcriptome remains mostly constant, while the proteome is being considerably adjusted throughout the embryonation. Threonine catalytic subunits of proteasome are constantly produced, while the cysteine peptidases prevailing in freshly laid eggs are supplemented by aspartic peptidases and metallopeptidases in the later stages of egg development.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA19-17269S" target="_blank" >GA19-17269S: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů