Direct Evidence for Crossover and Chromatid Interference in Meiosis of Two Plant Hybrids (Lolium Multiflorum × Festuca Pratensis And Allium Cepa × A. Roylei)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00120875" target="_blank" >RIV/00216224:14310/21:00120875 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1093/jxb/eraa455" target="_blank" >https://doi.org/10.1093/jxb/eraa455</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/eraa455" target="_blank" >10.1093/jxb/eraa455</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct Evidence for Crossover and Chromatid Interference in Meiosis of Two Plant Hybrids (Lolium Multiflorum × Festuca Pratensis And Allium Cepa × A. Roylei)

Popis výsledku v původním jazyce

Crossing over, apart from its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly controlled in frequency and distribution. The well-known crossover control is the positive crossover interference which reduces the probability of a crossover in the vicinity of an already formed crossover. A poorly studied aspect of the control is the chromatid interference. Such analyses are possible in very few organisms as they require observation of all four products of a single meiosis. Here, we provide direct evidence of chromatid interference. Using in situ probing in two interspecific plant hybrids (Lolium multiflorum × Festuca pratensis and Allium cepa × A. roylei) during anaphase I, we demonstrate that the involvement of four chromatids in double crossovers is significantly more frequent than expected (64% vs. 25%). We also provide a physical measure of the crossover interference distance, covering ca. 30-40% of the relative chromosome arm length, and show that the centromere acts as a barrier for crossover interference. The two arms of a chromosome appear to act as independent units in the process of crossing over. Chromatid interference has to be seriously addressed in genetic mapping approaches and further studies.

Název v anglickém jazyce

Direct Evidence for Crossover and Chromatid Interference in Meiosis of Two Plant Hybrids (Lolium Multiflorum × Festuca Pratensis And Allium Cepa × A. Roylei)

Popis výsledku anglicky

Crossing over, apart from its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly controlled in frequency and distribution. The well-known crossover control is the positive crossover interference which reduces the probability of a crossover in the vicinity of an already formed crossover. A poorly studied aspect of the control is the chromatid interference. Such analyses are possible in very few organisms as they require observation of all four products of a single meiosis. Here, we provide direct evidence of chromatid interference. Using in situ probing in two interspecific plant hybrids (Lolium multiflorum × Festuca pratensis and Allium cepa × A. roylei) during anaphase I, we demonstrate that the involvement of four chromatids in double crossovers is significantly more frequent than expected (64% vs. 25%). We also provide a physical measure of the crossover interference distance, covering ca. 30-40% of the relative chromosome arm length, and show that the centromere acts as a barrier for crossover interference. The two arms of a chromosome appear to act as independent units in the process of crossing over. Chromatid interference has to be seriously addressed in genetic mapping approaches and further studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

1460-2431

Svazek periodika

72

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

254-267

Kód UT WoS článku

000648886400010

EID výsledku v databázi Scopus

2-s2.0-85102929034