Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00125133" target="_blank" >RIV/00216224:14310/22:00125133 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61988987:17450/22:A2302E3T
Výsledek na webu
<a href="https://academic.oup.com/jcem/article/107/3/755/6406610" target="_blank" >https://academic.oup.com/jcem/article/107/3/755/6406610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1210/clinem/dgab756" target="_blank" >10.1210/clinem/dgab756</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
Popis výsledku v původním jazyce
Context: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. Objective: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. Methods: Abdominal subcutaneous (SA) and omental visceral adipocytes (VA) were isolated from paired adipose tissue biopsies obtained during bariatric surgery on 19 severely obese women (body mass index > 30 kg/m(2)) and analyzed using state-of-the-art mass spectrometry. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were performed to investigate proteome signature properties and to examine a possible association of the protein expression with the clinical data. Results: We identified 3686 protein groups and found 1140 differentially expressed proteins (adj. P value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. We provide a global protein profile of abdominal SA and omental VA, present the most differentially expressed pathways and processes distinguishing SA from VA, and correlate them with clinical and body composition data. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondria! energy metabolism and translational or biosynthetic activity is higher in VA. Conclusion: Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity.
Název v anglickém jazyce
Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
Popis výsledku anglicky
Context: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. Objective: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. Methods: Abdominal subcutaneous (SA) and omental visceral adipocytes (VA) were isolated from paired adipose tissue biopsies obtained during bariatric surgery on 19 severely obese women (body mass index > 30 kg/m(2)) and analyzed using state-of-the-art mass spectrometry. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were performed to investigate proteome signature properties and to examine a possible association of the protein expression with the clinical data. Results: We identified 3686 protein groups and found 1140 differentially expressed proteins (adj. P value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. We provide a global protein profile of abdominal SA and omental VA, present the most differentially expressed pathways and processes distinguishing SA from VA, and correlate them with clinical and body composition data. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondria! energy metabolism and translational or biosynthetic activity is higher in VA. Conclusion: Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30202 - Endocrinology and metabolism (including diabetes, hormones)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Clinical Endocrinology and Metabolism
ISSN
0021-972X
e-ISSN
1945-7197
Svazek periodika
107
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
21
Strana od-do
755-775
Kód UT WoS článku
000756897900013
EID výsledku v databázi Scopus
2-s2.0-85124850550