The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00127182" target="_blank" >RIV/00216224:14310/22:00127182 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

Popis výsledku v původním jazyce

Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.

Název v anglickém jazyce

The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

Popis výsledku anglicky

Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů