DNA barcoding the Lake Edward basin: high taxonomic coverage of a tropical freshwater ichthyofauna

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00128104" target="_blank" >RIV/00216224:14310/22:00128104 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s10750-022-04812-0" target="_blank" >https://doi.org/10.1007/s10750-022-04812-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10750-022-04812-0" target="_blank" >10.1007/s10750-022-04812-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA barcoding the Lake Edward basin: high taxonomic coverage of a tropical freshwater ichthyofauna

Popis výsledku v původním jazyce

We present an extensive DNA barcoding study of the fish species of the Lake Edward system, including information on intraspecific variation. The DNA barcode gene, cytochrome c oxidase I (COI), failed to discriminate the 35 species of the genus Haplochromis analysed. For the non-Haplochromis species, the reference library had a near complete coverage of 91.2%, with 31 out of the 34 known species of the system sequenced. With the recent morphology-based review of the ichthyofauna of the system as a backbone, the DNA barcoding library proved to be very effective for future species identifications. High identification successes were obtained based on Best Match (98.2%), Best Close Match (97.9%), and All Species Barcode (95.0%) criteria. Surprisingly, Laciris pelagicus and Micropanchax vitschumbaensis, two morphologically distinct species, had very similar and even identical COI haplotypes, whilst in Enteromius the existence of possible cryptic species was revealed. Few species occurring in the Lake Edward system were already represented in the global database Genbank. For some widespread species, regional genetic differences were found, highlighting the value of basin-specific information in reference databases. The provided reference library of DNA barcodes is a valuable tool for future conservation actions, which is certainly relevant in a system where the fisheries are under pressure.

Název v anglickém jazyce

DNA barcoding the Lake Edward basin: high taxonomic coverage of a tropical freshwater ichthyofauna

Popis výsledku anglicky

We present an extensive DNA barcoding study of the fish species of the Lake Edward system, including information on intraspecific variation. The DNA barcode gene, cytochrome c oxidase I (COI), failed to discriminate the 35 species of the genus Haplochromis analysed. For the non-Haplochromis species, the reference library had a near complete coverage of 91.2%, with 31 out of the 34 known species of the system sequenced. With the recent morphology-based review of the ichthyofauna of the system as a backbone, the DNA barcoding library proved to be very effective for future species identifications. High identification successes were obtained based on Best Match (98.2%), Best Close Match (97.9%), and All Species Barcode (95.0%) criteria. Surprisingly, Laciris pelagicus and Micropanchax vitschumbaensis, two morphologically distinct species, had very similar and even identical COI haplotypes, whilst in Enteromius the existence of possible cryptic species was revealed. Few species occurring in the Lake Edward system were already represented in the global database Genbank. For some widespread species, regional genetic differences were found, highlighting the value of basin-specific information in reference databases. The provided reference library of DNA barcodes is a valuable tool for future conservation actions, which is certainly relevant in a system where the fisheries are under pressure.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Hydrobiologia

ISSN

0018-8158

e-ISSN

1573-5117

Svazek periodika

849

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

20

Strana od-do

1743-1762

Kód UT WoS článku

000764987000001

EID výsledku v databázi Scopus

2-s2.0-85126035176