IPT9 promotes root growth

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F22%3A00128596" target="_blank" >RIV/00216224:14310/22:00128596 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

IPT9 promotes root growth

Popis výsledku v původním jazyce

Auxins and cytokinins are plant hormones that coordinate many aspects of plant development. Their interactions in plant underground growth are well established, occurring at the levels of metabolism, signaling, and transport. While most of multiple mutant lines, blocking specific parts of cytokinin biosynthetic pathways, have confirmed the impeding effect of cytokinin on root growth, the ipt29 double mutant instead surprisingly exhibits reduced primary root length compared to wild type. This mutant is impaired in cis-zeatin (cZ) production, a cytokinin-type that production follows tRNA degradation. We have investigated the intriguing ipt29 root phenotype, opposite to known cytokinin functions. Our data shows that despite cZ application can activate a cytokinin response in the root stele, it cannot rescue ipt29 short-root phenotype to WT. Grafting experiments revealed that the root phenotype of ipt29 depends mainly on local signaling which does not relate to directly to cytokinin levels. Notably, ipt29 displayed increased auxin levels in the root tissue. Moreover, analyses of the differential contributions of ipt2 and ipt9 to the ipt29 short-root phenotype demonstrated that, ipt2 does not show any root phenotype or auxin homeostasis variation while ipt9 mutants were indistinguishable from ipt29. We conclude that IPT9 functions may go beyond cZ biosynthesis, directly or indirectly, implicating effects on auxin homeostasis and therefore influencing root growth.

Název v anglickém jazyce

IPT9 promotes root growth

Popis výsledku anglicky

Auxins and cytokinins are plant hormones that coordinate many aspects of plant development. Their interactions in plant underground growth are well established, occurring at the levels of metabolism, signaling, and transport. While most of multiple mutant lines, blocking specific parts of cytokinin biosynthetic pathways, have confirmed the impeding effect of cytokinin on root growth, the ipt29 double mutant instead surprisingly exhibits reduced primary root length compared to wild type. This mutant is impaired in cis-zeatin (cZ) production, a cytokinin-type that production follows tRNA degradation. We have investigated the intriguing ipt29 root phenotype, opposite to known cytokinin functions. Our data shows that despite cZ application can activate a cytokinin response in the root stele, it cannot rescue ipt29 short-root phenotype to WT. Grafting experiments revealed that the root phenotype of ipt29 depends mainly on local signaling which does not relate to directly to cytokinin levels. Notably, ipt29 displayed increased auxin levels in the root tissue. Moreover, analyses of the differential contributions of ipt2 and ipt9 to the ipt29 short-root phenotype demonstrated that, ipt2 does not show any root phenotype or auxin homeostasis variation while ipt9 mutants were indistinguishable from ipt29. We conclude that IPT9 functions may go beyond cZ biosynthesis, directly or indirectly, implicating effects on auxin homeostasis and therefore influencing root growth.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/EF17_050%2F0008496" target="_blank" >EF17_050/0008496: MSCAfellow@MUNI</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů