Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00134152" target="_blank" >RIV/00216224:14310/23:00134152 - isvavai.cz</a>

Výsledek na webu

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202300152" target="_blank" >10.1002/jssc.202300152</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Popis výsledku v původním jazyce

Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.

Název v anglickém jazyce

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Popis výsledku anglicky

Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/GA19-08358S" target="_blank" >GA19-08358S: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

001017663100001

EID výsledku v databázi Scopus

2-s2.0-85163637022