Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00135361" target="_blank" >RIV/00216224:14310/23:00135361 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/23:43881259

Výsledek na webu

<a href="https://abs.bibl.u-szeged.hu/index.php/abs/article/view/3378" target="_blank" >https://abs.bibl.u-szeged.hu/index.php/abs/article/view/3378</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14232/abs.2023.1.35-44" target="_blank" >10.14232/abs.2023.1.35-44</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects

Popis výsledku v původním jazyce

Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates were included in the study, which were obtained from various geographical regions and environmental origins. Antimicrobial susceptibility testing was carried out using standard protocols. Biofilm-forming capacity and pyocyanin pigment production were tested using microtiter plate-based methods. Swarming, swimming and twitching motility, and siderophore-production were assessed using agar-plate based methodologies. Resistance in environmental isolates were highest for levofloxacin/ciprofloxacin 49.12% (n = 56), ceftazidime 42.98% (n = 49) and cefepime 35.96% (n = 41), while lowest for colistin 0% (n = 0); overexpression of RND-type efflux pumps was seen in 33.33% (n = 33) of isolates. 21.93% (n = 25) met the criteria to be classified as multidrug resistant (MDR). 17.54% (n = 20) of isolates were weak/non-biofilm producers, while (25.45%, n = 29) and (57.01%, n = 65) were moderate and strong biofilm producers, respectively. No significant differences were noted in biofilm-formation (OD570 values non-MDR [mean ± SD]: 0.396 ± 0.138 vs. MDR: 0.348 ± 0.181; p &gt; 0.05) or pyocyanin pigment production (OD686 values non-MDR: 0.403 ± 0.169 vs. MDR: 0.484 ± 0.125; p &gt; 0.05) between MDR and non-MDR environmental P. aeruginosa. Highest motility values were observed for swarming motility, followed by swimming and twitching motility; no relevant differences (p &gt; 0.05) in motility were noted in the context of MDR status or biofilm-formation in the tested isolates. P. aeruginosa is an opportunistic pathogen with high medical importance, being a causative agent of recalcitrant infections, which are becoming difficult to treat with the onset of MDR. Further studies are warranted to assess biofilm-forming capacity, and to provide insights into the mechanisms underlying biofilm-formation both in isolates of clinical and environmental origins.

Název v anglickém jazyce

Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects

Popis výsledku anglicky

Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates were included in the study, which were obtained from various geographical regions and environmental origins. Antimicrobial susceptibility testing was carried out using standard protocols. Biofilm-forming capacity and pyocyanin pigment production were tested using microtiter plate-based methods. Swarming, swimming and twitching motility, and siderophore-production were assessed using agar-plate based methodologies. Resistance in environmental isolates were highest for levofloxacin/ciprofloxacin 49.12% (n = 56), ceftazidime 42.98% (n = 49) and cefepime 35.96% (n = 41), while lowest for colistin 0% (n = 0); overexpression of RND-type efflux pumps was seen in 33.33% (n = 33) of isolates. 21.93% (n = 25) met the criteria to be classified as multidrug resistant (MDR). 17.54% (n = 20) of isolates were weak/non-biofilm producers, while (25.45%, n = 29) and (57.01%, n = 65) were moderate and strong biofilm producers, respectively. No significant differences were noted in biofilm-formation (OD570 values non-MDR [mean ± SD]: 0.396 ± 0.138 vs. MDR: 0.348 ± 0.181; p &gt; 0.05) or pyocyanin pigment production (OD686 values non-MDR: 0.403 ± 0.169 vs. MDR: 0.484 ± 0.125; p &gt; 0.05) between MDR and non-MDR environmental P. aeruginosa. Highest motility values were observed for swarming motility, followed by swimming and twitching motility; no relevant differences (p &gt; 0.05) in motility were noted in the context of MDR status or biofilm-formation in the tested isolates. P. aeruginosa is an opportunistic pathogen with high medical importance, being a causative agent of recalcitrant infections, which are becoming difficult to treat with the onset of MDR. Further studies are warranted to assess biofilm-forming capacity, and to provide insights into the mechanisms underlying biofilm-formation both in isolates of clinical and environmental origins.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Biologica Szegediensis

ISSN

1588-4082

e-ISSN

1588-385X

Svazek periodika

67

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

10

Strana od-do

35-44

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85187648807