Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00136352" target="_blank" >RIV/00216224:14310/24:00136352 - isvavai.cz</a>

Výsledek na webu

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202400157" target="_blank" >10.1002/jssc.202400157</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Popis výsledku v původním jazyce

CE is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterised by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility of performing chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilised onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilising in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilised for other biochemical systems such as affinity interaction studies or biochemically important processes such as cell lysis, even in single-cell format.

Název v anglickém jazyce

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Popis výsledku anglicky

CE is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterised by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility of performing chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilised onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilising in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilised for other biochemical systems such as affinity interaction studies or biochemically important processes such as cell lysis, even in single-cell format.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF SEPARATION SCIENCE

ISSN

1615-9306

e-ISSN

1615-9314

Svazek periodika

47

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

24

Strana od-do

1-24

Kód UT WoS článku

001268081000001

EID výsledku v databázi Scopus

2-s2.0-85197818193