Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00138856" target="_blank" >RIV/00216224:14310/24:00138856 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.respigbreed.cz/2024/2/1.pdf" target="_blank" >http://www.respigbreed.cz/2024/2/1.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia

Popis výsledku v původním jazyce

Ancient DNA (aDNA) is a DNA molecule older than 75 years. This molecule is characterized by many special properties. The main characteristics include its fragmentation. The short fragment length is often a limitation of the analysis. In this study, we analyzed historical animal samples from the Pohansko site (South Moravia, Czech Republic), which is dated to the 10th century. Two pig tooth samples were selected for aDNA extraction. After successful isolation, three nuclear DNA markers were amplified by PCR. These markers were selected due to the length of their amplification products (134 bp, 209 bp, and 312 bp, respectively). The amplification of the markers was successful for the HAL gene in one sample, and for the TEAD3 gene in both samples, the CYP2E1 gene was not successfully amplified in any sample.

Název v anglickém jazyce

Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia

Popis výsledku anglicky

Ancient DNA (aDNA) is a DNA molecule older than 75 years. This molecule is characterized by many special properties. The main characteristics include its fragmentation. The short fragment length is often a limitation of the analysis. In this study, we analyzed historical animal samples from the Pohansko site (South Moravia, Czech Republic), which is dated to the 10th century. Two pig tooth samples were selected for aDNA extraction. After successful isolation, three nuclear DNA markers were amplified by PCR. These markers were selected due to the length of their amplification products (134 bp, 209 bp, and 312 bp, respectively). The amplification of the markers was successful for the HAL gene in one sample, and for the TEAD3 gene in both samples, the CYP2E1 gene was not successfully amplified in any sample.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40200 - Animal and Dairy science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

18

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

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EID výsledku v databázi Scopus

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