Trematode Diplostomum pseudospathaceum inducing differential immune gene expression in sexual and gynogenetic gibel carp (Carassius gibelio), an invasive freshwater fish

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00139751" target="_blank" >RIV/00216224:14310/24:00139751 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Trematode Diplostomum pseudospathaceum inducing differential immune gene expression in sexual and gynogenetic gibel carp (Carassius gibelio), an invasive freshwater fish

Popis výsledku v původním jazyce

Diplostomum pseudospathaceum is a widely-distributed trematode parasite infecting the eyes of a wide range of the fish species. Gi¬bel carp (Carassius gibelio) may act as one of the potential second intermediate host. This invasive fish species often forms popula¬tions composed of sexual and asexual (reproducing by gynogene¬sis) specimens. We hypothesized that parasite-mediated selection is one of the potential mechanisms contributing to the coexistence of asexual-sexual complexes. The experimental infection of gy¬nogenetic and sexual gibel carp using D. pseudospathaceum was performed, and the transcriptome profile of the fish spleen was applied to reveal the differentially expressed immunity-associated genes related to D. pseudospathaceum infection. Gynogenetic fish were more parasitized when compared to sexuals. Our analy¬ses showed that eye trematode situated in an immune-privileged organ may induce an immune response in gibel carp. Differen¬tial gene expression induced by D. pseudospathaceum infection was revealed, with many genes documenting different impacts on gynogenetic and sexual gibel carp, specifically mostly DEGs upregulation in sexuals, and downregulation in asexuals. GO analyses revealed the importance of immune genes assigned to the following GO terms: immune function, the Notch signaling pathway, MAP kinase tyrosine/threonine/phosphatase activity, and chemokine receptor activity. The 12 immunity-associated pathways revealed by KEGG pathway enrichment analysis were affected by D. pseudospathaceum. Our study implies that para¬site-mediated selection and the modulation of organismal re¬sponses to parasite infection may represent the key mechanism contributing to the coexistence of asexual and sexual forms in gibel carp. We revealed the limited potential of asexual gibel carp to cope with parasite infection likely indicating a loss of capacity to induce an effective immune response.

Název v anglickém jazyce

Trematode Diplostomum pseudospathaceum inducing differential immune gene expression in sexual and gynogenetic gibel carp (Carassius gibelio), an invasive freshwater fish

Popis výsledku anglicky

Diplostomum pseudospathaceum is a widely-distributed trematode parasite infecting the eyes of a wide range of the fish species. Gi¬bel carp (Carassius gibelio) may act as one of the potential second intermediate host. This invasive fish species often forms popula¬tions composed of sexual and asexual (reproducing by gynogene¬sis) specimens. We hypothesized that parasite-mediated selection is one of the potential mechanisms contributing to the coexistence of asexual-sexual complexes. The experimental infection of gy¬nogenetic and sexual gibel carp using D. pseudospathaceum was performed, and the transcriptome profile of the fish spleen was applied to reveal the differentially expressed immunity-associated genes related to D. pseudospathaceum infection. Gynogenetic fish were more parasitized when compared to sexuals. Our analy¬ses showed that eye trematode situated in an immune-privileged organ may induce an immune response in gibel carp. Differen¬tial gene expression induced by D. pseudospathaceum infection was revealed, with many genes documenting different impacts on gynogenetic and sexual gibel carp, specifically mostly DEGs upregulation in sexuals, and downregulation in asexuals. GO analyses revealed the importance of immune genes assigned to the following GO terms: immune function, the Notch signaling pathway, MAP kinase tyrosine/threonine/phosphatase activity, and chemokine receptor activity. The 12 immunity-associated pathways revealed by KEGG pathway enrichment analysis were affected by D. pseudospathaceum. Our study implies that para¬site-mediated selection and the modulation of organismal re¬sponses to parasite infection may represent the key mechanism contributing to the coexistence of asexual and sexual forms in gibel carp. We revealed the limited potential of asexual gibel carp to cope with parasite infection likely indicating a loss of capacity to induce an effective immune response.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10613 - Zoology

Projekt

<a href="/cs/project/GA22-27023S" target="_blank" >GA22-27023S: Evoluční a ekologické mechanizmy koexistence asexuální a sexuální reprodukce u ryb</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů