Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00141099" target="_blank" >RIV/00216224:14310/25:00141099 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0301462225000559?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0301462225000559?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2025.107443" target="_blank" >10.1016/j.bpc.2025.107443</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine
Popis výsledku v původním jazyce
Benzo[c]phenanthridine alkaloids are known for their stabilizing effects on non-canonical DNA structures, particularly G-quadruplexes (G4s). In this study, the interaction of fagaronine, a rare benzo[c]phenanthridine alkaloid, with several DNA structures (including B-DNA, parallel, antiparallel and hybrid G4s) is studied using molecular fluorescence and circular dichroism (CD) spectroscopy. It has been found that fagaronine significantly enhances the stability of all tested G4 conformations. Furthermore, a study by NMR spectroscopy provided valuable information on the mechanism of interaction of the ligand with the parallel G4 structure adopted by Pu22T14T23, a sequence mutated with respect to that found within the promoter region of the c-myc gene. Remarkably, when compared with data reported in the literature, fagaronine appears to exhibit one of the strongest G4 thermal stabilization effects ever recorded for a small ligand.
Název v anglickém jazyce
Interaction process behind the strong stabilization of G-quadruplexes by alkaloid fagaronine
Popis výsledku anglicky
Benzo[c]phenanthridine alkaloids are known for their stabilizing effects on non-canonical DNA structures, particularly G-quadruplexes (G4s). In this study, the interaction of fagaronine, a rare benzo[c]phenanthridine alkaloid, with several DNA structures (including B-DNA, parallel, antiparallel and hybrid G4s) is studied using molecular fluorescence and circular dichroism (CD) spectroscopy. It has been found that fagaronine significantly enhances the stability of all tested G4 conformations. Furthermore, a study by NMR spectroscopy provided valuable information on the mechanism of interaction of the ligand with the parallel G4 structure adopted by Pu22T14T23, a sequence mutated with respect to that found within the promoter region of the c-myc gene. Remarkably, when compared with data reported in the literature, fagaronine appears to exhibit one of the strongest G4 thermal stabilization effects ever recorded for a small ligand.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10400 - Chemical sciences
Návaznosti výsledku
Projekt
<a href="/cs/project/EF18_046%2F0015974" target="_blank" >EF18_046/0015974: Modernizace České infrastruktury pro integrativní strukturní biologii</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biophysical Chemistry
ISSN
0301-4622
e-ISSN
1873-4200
Svazek periodika
323
Číslo periodika v rámci svazku
August 2025
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
12
Strana od-do
1-12
Kód UT WoS článku
001476223800001
EID výsledku v databázi Scopus
2-s2.0-105002677815