Quantification of soil microbial functional genes as potential new method in environmental risk assessment of pesticides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00142060" target="_blank" >RIV/00216224:14310/25:00142060 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10646-025-02920-w" target="_blank" >https://link.springer.com/article/10.1007/s10646-025-02920-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10646-025-02920-w" target="_blank" >10.1007/s10646-025-02920-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of soil microbial functional genes as potential new method in environmental risk assessment of pesticides

Popis výsledku v původním jazyce

Pesticides can have adverse effects on soil microorganisms, but they are underrepresented in the currently required OECD 216 test for environmental risk assessment of plant protection products (PPP). The guideline monitors soil microbial nitrogen transformation over 28 days, potentially missing long-term effects of persistent pesticides. Additionally, nitrate alone may be not sensitive enough to detect disruptions in microbial functions. We investigated whether functional gene analysis could provide a more sensitive bioindicator of pesticide impact. To compare this method with the standard test, we conducted a microcosm experiment following the OECD 216 experimental setup. To capture long-term effects beyond the typical test period, we extended the incubation duration to 56 days. Four different concentrations of the persistent fungicide boscalid were added based on predicted environmental concentration. We also assessed microbial responses to fungicide exposure by measuring classical soil microbial parameters. According to the standard test, boscalid had no harmful long-term effects on soil microbiota. In contrast, our analysis of functional genes found an overall reduction in the acid phosphatase-encoding phoN gene abundance on Day 56, and correspondingly, in acid phosphatase activity in the highest fungicide treatment. Simultaneously, we observed a tendency towards lower fungal abundance based on measured copy numbers of an ITS region of nuclear ribosomal DNA (rDNA) and increased cumulative CO2 production. These results indicate a fungicide-related response of the microbial community and impaired microbial phosphorus cycling. Extending the experimental period to 56 days revealed long-term effects that would have otherwise been undetected under the typical 28-day test duration.

Název v anglickém jazyce

Quantification of soil microbial functional genes as potential new method in environmental risk assessment of pesticides

Popis výsledku anglicky

Pesticides can have adverse effects on soil microorganisms, but they are underrepresented in the currently required OECD 216 test for environmental risk assessment of plant protection products (PPP). The guideline monitors soil microbial nitrogen transformation over 28 days, potentially missing long-term effects of persistent pesticides. Additionally, nitrate alone may be not sensitive enough to detect disruptions in microbial functions. We investigated whether functional gene analysis could provide a more sensitive bioindicator of pesticide impact. To compare this method with the standard test, we conducted a microcosm experiment following the OECD 216 experimental setup. To capture long-term effects beyond the typical test period, we extended the incubation duration to 56 days. Four different concentrations of the persistent fungicide boscalid were added based on predicted environmental concentration. We also assessed microbial responses to fungicide exposure by measuring classical soil microbial parameters. According to the standard test, boscalid had no harmful long-term effects on soil microbiota. In contrast, our analysis of functional genes found an overall reduction in the acid phosphatase-encoding phoN gene abundance on Day 56, and correspondingly, in acid phosphatase activity in the highest fungicide treatment. Simultaneously, we observed a tendency towards lower fungal abundance based on measured copy numbers of an ITS region of nuclear ribosomal DNA (rDNA) and increased cumulative CO2 production. These results indicate a fungicide-related response of the microbial community and impaired microbial phosphorus cycling. Extending the experimental period to 56 days revealed long-term effects that would have otherwise been undetected under the typical 28-day test duration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ECOTOXICOLOGY

ISSN

0963-9292

e-ISSN

1573-3017

Svazek periodika

34

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

1312-1324

Kód UT WoS článku

001518276900001

EID výsledku v databázi Scopus

2-s2.0-105009136110