Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00142583" target="_blank" >RIV/00216224:14310/25:00142583 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X25030851" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X25030851</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2025.115737" target="_blank" >10.1016/j.microc.2025.115737</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence

Popis výsledku v původním jazyce

The fluorescence properties of tetracycline antibiotics (tetracycline, minocycline, doxycycline, and omadacycline) were investigated in water and heavy water to evaluate the influence of solvent effects and the presence of metal ions on their spectral behavior. Emission spectra were recorded, both in the absence and presence of divalent metal cations (Mg2+, Ca2+) and the monovalent Li+ ion. All compounds exhibited enhanced fluorescence in D2O compared to H2O, with a marked increase upon the addition of Mg2+ ions. Emission maxima shifted in the presence of Mg2+, and fluorescence intensities reached up to 34.5-fold higher for omadacycline in D2O with Mg2+. Li+ showed no significant effect due to its weak coordination properties; however, fluorescence enhancement was still observed in D2O, indicating the sole influence of solvent even in the absence of strong complex formation. Chromatographic separation was successfully achieved for tetracycline, minocycline, and omadacycline using HPLC coupled with fluorescence detection. The combined use of D2O and Mg2+ not only improved detection sensitivity but also lowered limits of detection (LODs), reaching as low as 3.1 · 10−9 mol·L−1 for omadacycline. These findings highlight the cumulative effects of solvent and metal ion environments on tetracycline fluorescence and provide valuable insights for developing sensitive analytical methods for antibiotic detection in pharmaceutical, clinical, and environmental samples.

Název v anglickém jazyce

Lighting up tetracyclines: Heavy water solvent effects on antibiotic fluorescence

Popis výsledku anglicky

The fluorescence properties of tetracycline antibiotics (tetracycline, minocycline, doxycycline, and omadacycline) were investigated in water and heavy water to evaluate the influence of solvent effects and the presence of metal ions on their spectral behavior. Emission spectra were recorded, both in the absence and presence of divalent metal cations (Mg2+, Ca2+) and the monovalent Li+ ion. All compounds exhibited enhanced fluorescence in D2O compared to H2O, with a marked increase upon the addition of Mg2+ ions. Emission maxima shifted in the presence of Mg2+, and fluorescence intensities reached up to 34.5-fold higher for omadacycline in D2O with Mg2+. Li+ showed no significant effect due to its weak coordination properties; however, fluorescence enhancement was still observed in D2O, indicating the sole influence of solvent even in the absence of strong complex formation. Chromatographic separation was successfully achieved for tetracycline, minocycline, and omadacycline using HPLC coupled with fluorescence detection. The combined use of D2O and Mg2+ not only improved detection sensitivity but also lowered limits of detection (LODs), reaching as low as 3.1 · 10−9 mol·L−1 for omadacycline. These findings highlight the cumulative effects of solvent and metal ion environments on tetracycline fluorescence and provide valuable insights for developing sensitive analytical methods for antibiotic detection in pharmaceutical, clinical, and environmental samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

218

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

115737

Kód UT WoS článku

001603459600004

EID výsledku v databázi Scopus

2-s2.0-105018866276