Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F01%3A00004568" target="_blank" >RIV/00216224:14330/01:00004568 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.

Popis výsledku v původním jazyce

A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed i

Název v anglickém jazyce

Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.

Popis výsledku anglicky

A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed i

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA5004001" target="_blank" >IAA5004001: Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts

ISBN

80-7157-518-6

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

88

Název nakladatele

Mendel University of Agriculture and Forestry, Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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