Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes

Popis výsledku v původním jazyce

Nuclear location of subtelomeric and centromeric DNA sequences of chromosome 8, 9 and 13 was investigated in 3D fixed human G0 and stimulated T-lymphocytes using repeated dual colour FISH. In the first hybridisation, DNA probes for p and q arms labelledby different fluorochromes were used. The centromere and the chromosome territory of the same chromosome were visualised in the second hybridisation on the same nuclei. It was found for all investigated chromosomes that contrary to centromeres located inthe proximity of the nuclear membrane, the telomeres adopt more central position. These results indicate a hairpin like structure of chromosomes in the interphase nucleus. The results of this study provide information not only on the topography of subtelomeres but also images of the spatial arrangement and orientation of interphase chromosomes in cycling and non-cycling cell nuclei.

Název v anglickém jazyce

Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes

Popis výsledku anglicky

Nuclear location of subtelomeric and centromeric DNA sequences of chromosome 8, 9 and 13 was investigated in 3D fixed human G0 and stimulated T-lymphocytes using repeated dual colour FISH. In the first hybridisation, DNA probes for p and q arms labelledby different fluorochromes were used. The centromere and the chromosome territory of the same chromosome were visualised in the second hybridisation on the same nuclei. It was found for all investigated chromosomes that contrary to centromeres located inthe proximity of the nuclear membrane, the telomeres adopt more central position. These results indicate a hairpin like structure of chromosomes in the interphase nucleus. The results of this study provide information not only on the topography of subtelomeres but also images of the spatial arrangement and orientation of interphase chromosomes in cycling and non-cycling cell nuclei.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biophysics of the genome and its interactions

ISBN

80-210-2853-X

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

1

Název nakladatele

Masaryk University

Místo vydání

Brno

Místo konání akce

15-17.10.2001, Hlohovec

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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