Analysis of DNA methylation status of cell nuclei using high-resolution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F03%3A00009009" target="_blank" >RIV/00216224:14330/03:00009009 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14330/03:00008574

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of DNA methylation status of cell nuclei using high-resolution

Popis výsledku v původním jazyce

Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20. Sequential 5-methylcytosin (5-mC)-rich regions were visualised on metaphase chromosomes 1,10, 18, 19, and

Název v anglickém jazyce

Analysis of DNA methylation status of cell nuclei using high-resolution

Popis výsledku anglicky

Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20. Sequential 5-methylcytosin (5-mC)-rich regions were visualised on metaphase chromosomes 1,10, 18, 19, and

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAB5004102" target="_blank" >IAB5004102: Jaderná topologie některých protoonkogenů u lidských neutrofilních granulocytů a leukemických buněk</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biophysics of the Genome

ISBN

80-210-3226-X

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

59-61

Název nakladatele

Masaryk University

Místo vydání

Brno

Místo konání akce

Hlohovec, Czech Republic

Datum konání akce

1. 1. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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