Role INa a Ito ve frekvenční závislosti vlivu ajmalinu: analýza metodou vnuceného akčního napětí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00021293" target="_blank" >RIV/00216224:14330/04:00021293 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Role of INa and Ito in rate-dependent effect of ajmaline: action potential voltage clamp analysis

Popis výsledku v původním jazyce

The efficacy of antiarrhythmic drugs is significantly affected by their frequency dependent effect. This study was aimed to assess how Ca2+-independent currents contribute to the ajmaline-induced rate-dependent changes of action potential (AP) waveform in rat ventricular myocytes. To bring the analysis nearer to natural conditions, we used the AP voltage clamp method. In control, the gradual prolongation of AP correlated with gradual decrease of charge carried by transient outward current Ito measured in the AP voltage clamp mode. Under the effect of ajmaline, marked gradual decrease of AP amplitude correlated with gradual decrease of charge carried by sodium current INa. We conclude that INa, slowly recovering form ajmaline-induced block, can accountfor the gradual decrease of AP amplitude whereas the suppressed Ito was unable to induce significant changes of AP.

Název v anglickém jazyce

Role of INa and Ito in rate-dependent effect of ajmaline: action potential voltage clamp analysis

Popis výsledku anglicky

The efficacy of antiarrhythmic drugs is significantly affected by their frequency dependent effect. This study was aimed to assess how Ca2+-independent currents contribute to the ajmaline-induced rate-dependent changes of action potential (AP) waveform in rat ventricular myocytes. To bring the analysis nearer to natural conditions, we used the AP voltage clamp method. In control, the gradual prolongation of AP correlated with gradual decrease of charge carried by transient outward current Ito measured in the AP voltage clamp mode. Under the effect of ajmaline, marked gradual decrease of AP amplitude correlated with gradual decrease of charge carried by sodium current INa. We conclude that INa, slowly recovering form ajmaline-induced block, can accountfor the gradual decrease of AP amplitude whereas the suppressed Ito was unable to induce significant changes of AP.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

53

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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