Genomic Stability of the Cells during hiPSC Reprogramming and Endothelial Differentiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F16%3A00088049" target="_blank" >RIV/00216224:14330/16:00088049 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genomic Stability of the Cells during hiPSC Reprogramming and Endothelial Differentiation

Popis výsledku v původním jazyce

Studies of endothelial biology at genetic and molecular level are limited by the availability of relevant endothelial cells (ECs). Human induced pluripotent stem cells (hiPSCs) offer a potentially unlimited source of ECs. hiPSC-derived ECs (hiPSC-ECs) provide a robust and reproducible patient-specific model system for (1) tissue engineering, (2) drug development, (3) toxicity screening, and (4) disease modeling. However, one of the main concerns is the maintenance of genomic integrity of the cells throughout the processes of hiPSC reprogramming and differentiation in vitro. In our study we generated hiPSCs from various cell-type sources, including human umbilical vein ECs (HUVECs) and adult vein ECs. After phenotypical and functional characterization, hiPSC lines were differentiated into the hiPSC-ECs using previously published protocol (Orlova et al., 2014). Upon magnetic beads-based purification, the hiPSC-EC population displayed endothelial surface markers and functions consistent with primary ECs.

Název v anglickém jazyce

Genomic Stability of the Cells during hiPSC Reprogramming and Endothelial Differentiation

Popis výsledku anglicky

Studies of endothelial biology at genetic and molecular level are limited by the availability of relevant endothelial cells (ECs). Human induced pluripotent stem cells (hiPSCs) offer a potentially unlimited source of ECs. hiPSC-derived ECs (hiPSC-ECs) provide a robust and reproducible patient-specific model system for (1) tissue engineering, (2) drug development, (3) toxicity screening, and (4) disease modeling. However, one of the main concerns is the maintenance of genomic integrity of the cells throughout the processes of hiPSC reprogramming and differentiation in vitro. In our study we generated hiPSCs from various cell-type sources, including human umbilical vein ECs (HUVECs) and adult vein ECs. After phenotypical and functional characterization, hiPSC lines were differentiated into the hiPSC-ECs using previously published protocol (Orlova et al., 2014). Upon magnetic beads-based purification, the hiPSC-EC population displayed endothelial surface markers and functions consistent with primary ECs.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů