Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14410%2F04%3A00030654" target="_blank" >RIV/00216224:14410/04:00030654 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes
Popis výsledku v původním jazyce
Polynuclear platinum compounds demonstrate many novel phenomena in their interactions with DNA and proteins as well as novel anti-cancer activities. Previous studies indicated that the high positive charge and the non-coordinated Ącentral linkerĄą of thepolynuclear compounds could have major contributions to these features. Therefore, a series of non-covalent polynuclear platinum complexes, [ Pt(NH3)3 2-ŚĚ-Y]n+ (Y = polyamine linker or trans-Pt(NH3)2(H2N(CH2)6NH2)2 ) was synthesized and the DNA interactions of these platinum complexes were investigated. The conformational changes induced by these compounds in polymer DNA were studied by circular dichroism and the reversibility of the transition was tested by subsequent titration with the DNA intercalating agent ethidium bromide (EtBr). Fluorescent quenching was also used to assess the ability of EtBr to intercalate into A and Z-DNA induced by the compounds.
Název v anglickém jazyce
Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes
Popis výsledku anglicky
Polynuclear platinum compounds demonstrate many novel phenomena in their interactions with DNA and proteins as well as novel anti-cancer activities. Previous studies indicated that the high positive charge and the non-coordinated Ącentral linkerĄą of thepolynuclear compounds could have major contributions to these features. Therefore, a series of non-covalent polynuclear platinum complexes, [ Pt(NH3)3 2-ŚĚ-Y]n+ (Y = polyamine linker or trans-Pt(NH3)2(H2N(CH2)6NH2)2 ) was synthesized and the DNA interactions of these platinum complexes were investigated. The conformational changes induced by these compounds in polymer DNA were studied by circular dichroism and the reversibility of the transition was tested by subsequent titration with the DNA intercalating agent ethidium bromide (EtBr). Fluorescent quenching was also used to assess the ability of EtBr to intercalate into A and Z-DNA induced by the compounds.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
BO - Biofyzika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2004
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Inorganic Biochemistry
ISSN
0162-0134
e-ISSN
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Svazek periodika
98
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
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Kód UT WoS článku
000224599100007
EID výsledku v databázi Scopus
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