Extending the linear range of the SALDI-MS analyses using the internal standard technique

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F12%3A00057867" target="_blank" >RIV/00216224:14740/12:00057867 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Extending the linear range of the SALDI-MS analyses using the internal standard technique

Popis výsledku v původním jazyce

Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) employing gold nanoparticles (Au NPs) has been presented as a useful method for the both qualitative and quantitative analysis of low molecular weight analytes. Besides the well-known benefit, such as the improvement in the analysis precision, the use of an internal standard was observed to significantly extend the linear range of SALDI MS. Without the use of the internal standard, the linear range is presumably limited by the saturation of the Au NP surface where the desorption process takes place: at higher analyte concentration, an imperfect, multilayer assembly is formed leading to inefficient desorption and/or ionization and thus to reduced linearity and poor reproducibility.

Název v anglickém jazyce

Extending the linear range of the SALDI-MS analyses using the internal standard technique

Popis výsledku anglicky

Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) employing gold nanoparticles (Au NPs) has been presented as a useful method for the both qualitative and quantitative analysis of low molecular weight analytes. Besides the well-known benefit, such as the improvement in the analysis precision, the use of an internal standard was observed to significantly extend the linear range of SALDI MS. Without the use of the internal standard, the linear range is presumably limited by the saturation of the Au NP surface where the desorption process takes place: at higher analyte concentration, an imperfect, multilayer assembly is formed leading to inefficient desorption and/or ionization and thus to reduced linearity and poor reproducibility.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů