Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00065831" target="_blank" >RIV/00216224:14740/13:00065831 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6" target="_blank" >http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-333-6_2" target="_blank" >10.1007/978-1-62703-333-6_2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Popis výsledku v původním jazyce

Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.

Název v anglickém jazyce

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Popis výsledku anglicky

Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Meiosis: Methods and Protocols

ISBN

9781627033329

Počet stran výsledku

12

Strana od-do

13-24

Počet stran knihy

238

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

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