Off-line MALDI and ICP MS detection of a single record of metallothionein separation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00066028" target="_blank" >RIV/00216224:14740/13:00066028 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Off-line MALDI and ICP MS detection of a single record of metallothionein separation

Popis výsledku v původním jazyce

It is estimated that one third of all proteins require metals to carry out their functions. Therefore, the analyses of native metal-protein and metal-metabolite complexes by hyphenated techniques that combine separation with a specific detection is of growing importance. A new concept of an off-line multidetection platform for metalloprotein/metallopeptide analysis of fractions collected from a single run of capillary electrophoresis (CE) or liquid chromatography (LC) is presented. The separation record, i.e. fractions collected on a target, are analyzed sequentially with MALDI MS and SALD ICP MS. Thus, a single separation can yield both proteomic and metallomic information. Moreover, the off-line nature of the analysis provides further possibilities,such as laser-induced fluorescence detection or on target digestion.

Název v anglickém jazyce

Off-line MALDI and ICP MS detection of a single record of metallothionein separation

Popis výsledku anglicky

It is estimated that one third of all proteins require metals to carry out their functions. Therefore, the analyses of native metal-protein and metal-metabolite complexes by hyphenated techniques that combine separation with a specific detection is of growing importance. A new concept of an off-line multidetection platform for metalloprotein/metallopeptide analysis of fractions collected from a single run of capillary electrophoresis (CE) or liquid chromatography (LC) is presented. The separation record, i.e. fractions collected on a target, are analyzed sequentially with MALDI MS and SALD ICP MS. Thus, a single separation can yield both proteomic and metallomic information. Moreover, the off-line nature of the analysis provides further possibilities,such as laser-induced fluorescence detection or on target digestion.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů