Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00075044" target="_blank" >RIV/00216224:14740/13:00075044 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.pnas.org/content/110/9/3627" target="_blank" >http://www.pnas.org/content/110/9/3627</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.1300107110" target="_blank" >10.1073/pnas.1300107110</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism

Popis výsledku v původním jazyce

Gravitropic bending of plant organs is mediated by an asymmetric signaling of the plant hormone auxin between the upper and lower side of the respective organ. Here, we show that also another plant hormone, gibberellie acid (GA), shows asymmetric actionduring gravitropic responses. lmmunodetection using an antibody against GA and monitoring GA signaling output by downstream degradation of DELLA proteins revealed an asymmetric GA distribution and response with the maximum at the lower side of gravistimulated roots. Genetic or pharmacological manipulation of GA levels or response affects gravity-mediated auxin redistribution and root bending response. The higher GA levels at the lower side of the root correlate with increased amounts of PIN-FORMED2 (PIN2) auxin transporter at the plasma membrane. The observed increase in PIN2 stability is caused by a specific GA effect on trafficking of PIN proteins to lytic vacuoles that presumably occurs downstream of brefeldin A-sensitive endosomes.

Název v anglickém jazyce

Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism

Popis výsledku anglicky

Gravitropic bending of plant organs is mediated by an asymmetric signaling of the plant hormone auxin between the upper and lower side of the respective organ. Here, we show that also another plant hormone, gibberellie acid (GA), shows asymmetric actionduring gravitropic responses. lmmunodetection using an antibody against GA and monitoring GA signaling output by downstream degradation of DELLA proteins revealed an asymmetric GA distribution and response with the maximum at the lower side of gravistimulated roots. Genetic or pharmacological manipulation of GA levels or response affects gravity-mediated auxin redistribution and root bending response. The higher GA levels at the lower side of the root correlate with increased amounts of PIN-FORMED2 (PIN2) auxin transporter at the plasma membrane. The observed increase in PIN2 stability is caused by a specific GA effect on trafficking of PIN proteins to lytic vacuoles that presumably occurs downstream of brefeldin A-sensitive endosomes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proc. Natl. Acad. Sci. U. S. A.

ISSN

0027-8424

e-ISSN

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Svazek periodika

110

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

3627-3632

Kód UT WoS článku

000315841900083

EID výsledku v databázi Scopus

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