In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073698" target="_blank" >RIV/00216224:14740/14:00073698 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/14:00429937

Výsledek na webu

<a href="http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm" target="_blank" >http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1042/BST20130204" target="_blank" >10.1042/BST20130204</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

Popis výsledku v původním jazyce

The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), toperform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential malecomponents in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secr

Název v anglickém jazyce

In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

Popis výsledku anglicky

The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), toperform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential malecomponents in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOCHEMICAL SOCIETY TRANSACTIONS

ISSN

0300-5127

e-ISSN

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Svazek periodika

42

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

388-394

Kód UT WoS článku

000333658900028

EID výsledku v databázi Scopus

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