New sensitive detection method for lectin hemagglutination using microscopy.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073809" target="_blank" >RIV/00216224:14740/14:00073809 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jemt.22407" target="_blank" >http://dx.doi.org/10.1002/jemt.22407</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jemt.22407" target="_blank" >10.1002/jemt.22407</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New sensitive detection method for lectin hemagglutination using microscopy.

Popis výsledku v původním jazyce

The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope.

Název v anglickém jazyce

New sensitive detection method for lectin hemagglutination using microscopy.

Popis výsledku anglicky

The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy research and technique

ISSN

1059-910X

e-ISSN

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Svazek periodika

77

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

841-849

Kód UT WoS článku

000342207900012

EID výsledku v databázi Scopus

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